Product Info Summary
SKU: | PT0005 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody
SKU/Catalog Number
PT0005
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody catalog # PT0005. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PT0005)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAOI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from Phosphotyrosine
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
PT0005 is reactive to Tyrosine in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
140 kDa, 130 kDa, 110 kDa
Calculated molecular weight
13989 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PT0005 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: Jurkat cell
ICC/IF: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phosphotyrosine expression in Jurkat cell lysate.
Click image to see more details
Immunofluorescent analysis of A431 cells treated with pervanadate, using Phosphotyrosine Antibody.
Protein Target Info & Infographic
Gene/Protein Information For Tyrosine (Source: Uniprot.org, NCBI)
Gene Name
Tyrosine
Full Name
Weight
13989 MW
Alternative Names
Histone H2A.2;HTA2;H2A2;YBL003C;YBL0103;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tyrosine, check out the Tyrosine Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tyrosine: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Tyrosine Rabbit Monoclonal Antibody (PT0005)
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