Click image to see more details
Product Info Summary
| SKU: | P00026 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00026
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody catalog # P00026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FOC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-c-Myc (T58)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00026 is reactive to MYC in Human
Reconstitution
Observed Molecular Weight
65 kDa
Calculated molecular weight
48804 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00026 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell, HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-c-Myc (T58) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin A and Okadaic Acid.
Protein Target Info & Infographic
Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)
Gene Name
MYC
Full Name
Myc proto-oncogene protein
Weight
48804 MW
Alternative Names
Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64;MYC;BHLHE39; MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYC, check out the MYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody (P00026)
Hello CJ!
P00026 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression
Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2
Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to literature, cervix carcinoma does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-21
Question
We have tried in the past anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody for WB on placenta testis a few months ago. I am using human, and We intend to use the antibody for IF next. My lab would like examining placenta testis as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
I have checked the website and datasheets of our anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody and I see that P00026 has been validated on human in both WB and IF. Thus P00026 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use using your anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody for positive regulation of telomerase activity studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
Thank you for your inquiry. This P00026 anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-08
Question
We have observed staining in human thoracic mammary gland. Are there any suggestions? Is anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody supposed to stain thoracic mammary gland positively?
B. Jones
Verified customer
Asked: 2016-09-21
Answer
From literature thoracic mammary gland does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-09-21
Question
We are currently using anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody P00026 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
D. Zhao
Verified customer
Asked: 2015-11-10
Answer
The anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody (P00026) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-10
