Product Info Summary
SKU: | M03065 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-PHF6 Antibody (C-Term)
SKU/Catalog Number
M03065
Size
50 µl
Description
Boster Bio Anti-PHF6 Antibody (C-Term) (Catalog # M03065). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PHF6 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03065)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PHF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 315-349 amino acids from human PHF6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03065 is reactive to PHF6 in Human
Reconstitution
Calculated molecular weight
41290 Da
Background of Phf6
Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03065 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PHF6 with M03065 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
Click image to see more details
All lanes : Anti-PHF6 Antibody (C-Term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For PHF6 (Source: Uniprot.org, NCBI)
Gene Name
PHF6
Full Name
PHD finger protein 6
Weight
41290 Da
Alternative Names
PHD finger protein 6, PHD-like zinc finger protein, PHF6, CENP-31, KIAA1823 Phf6|2700007B13Rik, 4931428F02Rik, mKIAA1823|PHD finger protein 6|PHD finger protein 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHF6, check out the PHF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHF6 Antibody (C-Term) (M03065)
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