Anti-PHF6 Antibody (C-Term)

Product Name

Anti-PHF6 Antibody (C-Term)

View all Phf6 Antibodies

SKU/Catalog Number

M03065

Size

50 µl

Description

Boster Bio Anti-PHF6 Antibody (C-Term) (Catalog # M03065). Tested in WB, IF application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PHF6 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03065)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This PHF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 315-349 amino acids from human PHF6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03065 is reactive to Phf6 in Human

Applications

M03065 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41290 Da

Background of Phf6

Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:2000

Validation Images & Assay Conditions

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PHF6 with M03065 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).

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All lanes : Anti-PHF6 Antibody (C-Term) at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For Phf6 (Source: Uniprot.org, NCBI)

Alternative Names

BFLS; BORJ; Borjeson-Forssman-Lehmann syndrome; KIAA1823; MGC14797; PHD finger protein 6; PHD-like zinc finger protein Phf6|2700007B13Rik, 4931428F02Rik, mKIAA1823|PHD finger protein 6|PHD finger protein 6

For more info on Phf6, check out the Phf6 Infographic

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In this infographic, you will see the following information for Phf6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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