Anti-PHACTR1 Antibody Picoband™

Product Name

Anti-PHACTR1 Antibody Picoband™

View all Phactr1 Antibodies

SKU/Catalog Number

A06344-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PHACTR1 Antibody Picoband™ catalog # A06344-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PHACTR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06344-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human PHACTR1 recombinant protein (Position: K66-E395). Human PHACTR1 shares 94.8% and 95.2% amino acid (aa) sequence identity with mouse and rat PHACTR1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06344-1 is reactive to Phactr1 in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

Calculated molecular weight

Background of Phactr1

Phosphatase and actin regulator 1 (PHACTR1) is a protein that in humans is encoded by the PHACTR1 gene on chromosome 6. The protein encoded by this gene is a member of the phosphatase and actin regulator family of proteins. This family member can bind actin and regulate the reorganization of the actin cytoskeleton. It plays a role in tubule formation and in endothelial cell survival. Polymorphisms in this gene are associated with susceptibility to myocardial infarction, coronary artery disease and cervical artery dissection. Alternative splicing of this gene results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06344-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PHACTR1 using anti-PHACTR1 antibody (A06344-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHACTR1 antigen affinity purified polyclonal antibody (Catalog # A06344-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHACTR1 at approximately 69 kDa. The expected band size for PHACTR1 is at 66 kDa.

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Figure 2. Flow Cytometry analysis of A549 cells using anti-PHACTR1 antibody (A06344-1).
Overlay histogram showing A549 cells stained with A06344-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHACTR1 Antibody (A06344-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Phactr1 (Source: Uniprot.org, NCBI)

Alternative Names

dJ257A7.2; KIAA1733MGC126577; MGC126575; phosphatase and actin regulator 1; RPEL repeat containing 1; RPEL; RPEL1 Phactr1|9630030F18Rik, Rp, Rpel1|phosphatase and actin regulator 1|phosphatase and actin regulator 1|RPEL repeat containing 1

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In this infographic, you will see the following information for Phactr1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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