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Product Info Summary
| SKU: | A09568 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PGLYRP2 Antibody Picoband®
View all PGLYRP2/PGRP-L Antibodies
SKU/Catalog Number
A09568
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PGLYRP2 Antibody Picoband® catalog # A09568. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PGLYRP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09568)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PGLYRP2 recombinant protein (Position: D198-Q524). Human PGLYRP2 shares 79.2% amino acid (aa) sequence identity with mouse PGLYRP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09568 is reactive to PGLYRP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
74 kDa
Calculated molecular weight
57.707kDa
Background of PGLYRP2/PGRP-L
Peptidoglycan recognition protein 2 (PGLYRP2) is an enzyme (EC 3.5.1.28), N-acetylmuramoyl-L-alanine amidase (NAMLAA), that hydrolyzes bacterial cell wall peptidoglycan and is encoded by the PGLYRP2 gene. This gene encodes a peptidoglycan recognition protein, which belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. This protein hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in bacterial cell wall glycopeptides, and thus may play a scavenger role by digesting biologically active peptidoglycan into biologically inactive fragments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09568 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human HepG2 whole cell, human RT4 whole cell, human K562 whole cell, rat liver tissue, rat RH-35 whole cell, mouse liver tissue, mouse Hepa1-6 whole cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PGLYRP2 using anti-PGLYRP2 antibody (A09568).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLYRP2 antigen affinity purified polyclonal antibody (Catalog # A09568) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGLYRP2 at approximately 74 kDa. The expected band size for PGLYRP2 is at 62 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-PGLYRP2 antibody (A09568).
Overlay histogram showing K562 cells stained with A09568 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PGLYRP2 Antibody (A09568, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PGLYRP2 (Source: Uniprot.org, NCBI)
Gene Name
PGLYRP2
Full Name
N-acetylmuramoyl-L-alanine amidase
Weight
57.707kDa
Superfamily
N-acetylmuramoyl-L-alanine amidase 2 family
Alternative Names
EC 3.5.1.28; peptidoglycan recognition protein 2tagl-beta; peptidoglycan recognition protein L; PGLYRP2; PGLYRPL; PGLYRPLHMFT0141; PGRP2; PGRPL; PGRP-L; PGRP-LN-acetylmuramoyl-L-alanine amidase; PGRPLPeptidoglycan recognition protein long; TAGL; tagL-alpha; TAGL-like Pglyrp2|C730002N09Rik, PG, PGRP-L, Pglyrpl, ta, tagL, tagl-b|peptidoglycan recognition protein 2|N-acetylmuramoyl-L-alanine amidase|peptidoglycan recognition protein long
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PGLYRP2, check out the PGLYRP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PGLYRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGLYRP2 Antibody Picoband® (A09568)
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