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Product Info Summary
| SKU: | A02605-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PDE10A Antibody Picoband®
SKU/Catalog Number
A02605-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDE10A Antibody Picoband® catalog # A02605-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDE10A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02605-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDE10A recombinant protein (Position: H515-D779).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02605-2 is reactive to PDE10A in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
35487 MW
Background of PDE10A
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02605-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse brain tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PDE10A using anti-PDE10A antibody (A02605-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE10A antigen affinity purified polyclonal antibody (Catalog # A02605-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE10A at approximately 100KD. The expected band size for PDE10A is at 100KD.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-PDE10A antibody (A02605-2).
Overlay histogram showing A549 cells stained with A02605-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE10A Antibody (A02605-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PDE10A (Source: Uniprot.org, NCBI)
Gene Name
PDE10A
Full Name
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Weight
35487 MW
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
Homeobox protein SIX3;Sine oculis homeobox homolog 3;SIX3; PDE10A ADSD2, HSPDE10A, IOLOD, LINC0047319, PDE10A phosphodiesterase 10A cAMP and cAMP-inhibited cGMP 3,5-cyclic phosphodiesterase 10A|3,5 cAMP/cGMP phosphodiesterase|dJ416F21.1 (phosphodiesterase 10A)|phosphodiesterase 10A1 (PDE10A1)|phosphodiesterase 10A7 (PDE10A7)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE10A, check out the PDE10A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE10A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE10A Antibody Picoband® (A02605-2)
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