Anti-PATZ1 Antibody Picoband™

Product Name

Anti-PATZ1 Antibody Picoband™

View all PATZ Antibodies

SKU/Catalog Number

A06823-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PATZ1 Antibody Picoband™ catalog # A06823-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PATZ1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06823-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human PATZ1 recombinant protein (Position: M145-Q680). Human PATZ1 shares 98.9% amino acid (aa) sequence identity with mouse PATZ1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06823-1 is reactive to PATZ1 in Human

Applications

A06823-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

52588 MW

Background of PATZ

POZ-, AT hook-, and zinc finger-containing protein 1 is a protein that in humans is encoded by the PATZ1 gene. The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PATZ1 using anti-PATZ1 antibody (A06823-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PATZ1 antigen affinity purified polyclonal antibody (Catalog # A06823-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PATZ1 at approximately 70 kDa. The expected band size for PATZ1 is at 74 kDa.

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Figure 2. IF analysis of PATZ1 using anti-PATZ1 antibody (A06823-1) and anti-Beta Tubulin antibody (M01857-3).
PATZ1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PATZ1 Antibody (A06823-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of Hela cells using anti-PATZ1 antibody (A06823-1).
Overlay histogram showing Hela cells stained with A06823-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PATZ1 Antibody (A06823-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PATZ1 (Source: Uniprot.org, NCBI)

Alternative Names

dJ400N23; MAZRPOZ-, AT hook-, and zinc finger-containing protein 1; PATZZinc finger and BTB domain-containing protein 19; POZ (BTB) and AT hook containing zinc finger 1; Protein kinase A RI subunit alpha-associated protein; RIAZBTB/POZ domain zinc finger transcription factor; ZBTB19BTB-POZ domain zinc finger transcription factor; Zinc finger protein 278Zinc finger sarcoma gene protein; ZNF278POZ-AT hook-zinc finger protein; ZSGMAZ-related factor PATZ1 MAZR, PATZ, RIAZ, ZBTB19, ZNF278, ZSG, dJ400N23 POZ/BTB and AT hook containing zinc finger 1 POZ-, AT hook-, and zinc finger-containing protein 1|BTB-POZ domain zinc finger transcription factor|MAZ-related factor|POZ-AT hook-zinc finger protein|protein kinase A RI subunit alpha-associated protein|zinc finger and BTB domain-containing protein 19|zinc finger protein 278|zinc finger sarcoma gene protein

For more info on PATZ1, check out the PATZ1 Infographic

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In this infographic, you will see the following information for PATZ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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