Anti-p28 ING5 Antibody

Ing5 antibody

Boster Bio Anti-p28 ING5 Antibody (Catalog # A04974). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A04974
Size: 100ug
Reactive Species: Human
Host: Goat
Application: ELISA, WB

Product Name

Anti-p28 ING5 Antibody

View all Ing5 Antibodies

SKU/Catalog Number

A04974

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p28 ING5 Antibody (Catalog # A04974). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-p28 ING5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04974)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04974 is reactive to ING5 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

27751 MW

Background of Ing5

p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) encodes the longest isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04974 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:16,000
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For ING5 (Source: Uniprot.org, NCBI)

Gene Name

ING5

Full Name

Inhibitor of growth protein 5

Weight

27751 MW

Superfamily

ING family

Alternative Names

p28 ING5, p28ING5, ING-5, ING 5, Inhibitor of growth family member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ING5, check out the ING5 Infographic

ING5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ING5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-p28 ING5 Antibody

Question

We are currently using anti-p28 ING5 antibody A04974 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

K. Carter

Verified customer

Asked: 2016-01-08

Answer

The anti-p28 ING5 antibody (A04974) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-01-08

Order DetailsPrice
A04974

100 μg

$629

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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