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Product Info Summary
| SKU: | A05141-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-OXSR1 Antibody Picoband®
SKU/Catalog Number
A05141-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OXSR1 Antibody Picoband® catalog # A10973-2. Tested in WB, IP, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OXSR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05141-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human OXSR1 recombinant protein (Position: K351-S527). Human OXSR1 shares 89.8% and 85.4% amino acid (aa) sequence identity with mouse and rat OXSR1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05141-2 is reactive to OXSR1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
52588 MW
Background of OXSR1
Serine/threonine-protein kinase OSR1 is an enzyme that in humans is encoded by the OXSR1 gene. The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05141-2 is guaranteed for ELISA, Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell, human Jurkat whole cell, human U251 whole cell, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue
IP: U251 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (Catalog # A05141-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-OXSR1 antibody (A05141-2).
Overlay histogram showing MCF-7 cells stained with A05141-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSR1 Antibody (A05141-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 3. Immunoprecipitating OXSR1 in U251 whole cell lysate .
Western blot analysis of OXSR1 using anti-OXSR1 antibody (A05141-2).
Lane 1: U251 whole cell lysates (30ug)
Lane 2: Rabbit control IgG instead of anti-OXSR1 antibody in U251 whole cell lysate.
Lane 3: anti-OXSR1 antibody (2μg) + U251 whole cell lysate (500μg)
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OXSR1 antigen affinity purified polyclonal antibody (A05141-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for OXSR1 at approximately 58 kDa. The expected band size for OXSR1 is at 58 kDa.
Protein Target Info & Infographic
Gene/Protein Information For OXSR1 (Source: Uniprot.org, NCBI)
Gene Name
OXSR1
Full Name
Serine/threonine-protein kinase OSR1
Weight
52588 MW
Superfamily
protein kinase superfamily
Alternative Names
70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody OXSR1 OSR1 oxidative stress responsive kinase 1 serine/threonine-protein kinase OSR1|oxidative stress responsive 1|oxidative stress-responsive 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OXSR1, check out the OXSR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OXSR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OXSR1 Antibody Picoband® (A05141-2)
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