Anti-OTUD3 Antibody Picoband™

Product Name

Anti-OTUD3 Antibody Picoband™

View all OTUD3 Antibodies

SKU/Catalog Number

A12734

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-OTUD3 Antibody Picoband™ catalog # A06478-1. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OTUD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12734)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human OTUD3 recombinant protein (Position: E51-I398). Human OTUD3 shares 82.8% amino acid (aa) sequence identity with mouse OTUD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A12734 is reactive to OTUD3 in Human

Applications

A12734 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

52588 MW

Background of OTUD3

OTUD3, a deubiquitinating enzyme, has emerged as important role in some cancer. It showed that OTUD3 plays suppressive role in breast cancer whereas oncogenic role in lung cancer. However, the function and mechanism of OTUD3 in hepatocellular carcinoma (HCC) progression remain elusive.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of OTUD3 using anti-OTUD3 antibody (A12734).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD3 antigen affinity purified polyclonal antibody (Catalog # A12734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD3 at approximately 43 kDa. The expected band size for OTUD3 is at 45 kDa.

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Figure 2. IF analysis of OTUD3 using anti-OTUD3 antibody (A12734).
OTUD3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OTUD3 Antibody (A12734) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For OTUD3 (Source: Uniprot.org, NCBI)

Alternative Names

OTU domain-containing protein 3 OTUD3 DUBA4 OTU deubiquitinase 3 OTU domain-containing protein 3|OTU domain containing 3

For more info on OTUD3, check out the OTUD3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for OTUD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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