Product Info Summary
SKU: | M04407 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Oct-2 Monoclonal Antibody
SKU/Catalog Number
M04407
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Oct-2 Monoclonal Antibody catalog # M04407. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oct-2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04407)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEBG-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Oct-2 Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') . Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04407 is reactive to POU2F2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
18930 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04407 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Oct-2 expression in Ramos cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For POU2F2 (Source: Uniprot.org, NCBI)
Gene Name
POU2F2
Full Name
POU domain, class 2, transcription factor 2
Weight
18930 MW
Superfamily
POU transcription factor family
Alternative Names
T-cell surface glycoprotein CD3 delta chain;T-cell receptor T3 delta chain;CD3d;CD3D;T3D; POU2F2 OCT2, OTF2, Oct-2 POU class 2 homeobox 2 POU domain, class 2, transcription factor 2|OTF-2|homeobox protein|lymphoid-restricted immunoglobulin octamer-binding protein NF-A2|octamer-binding protein 2|octamer-binding transcription factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POU2F2, check out the POU2F2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POU2F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Oct-2 Monoclonal Antibody (M04407)
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Customer Q&As
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1 Customer Q&As for Anti-Oct-2 Monoclonal Antibody
Question
We are currently using anti-Oct-2 Monoclonal antibody M04407 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
W. Lewis
Verified customer
Asked: 2020-02-14
Answer
The anti-Oct-2 Monoclonal antibody (M04407) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-14