Click image to see more details
Product Info Summary
| SKU: | A12282-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-OC90 Antibody Picoband®
SKU/Catalog Number
A12282-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OC90 Antibody Picoband® catalog # A12282-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OC90 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12282-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OC90 recombinant protein (Position: H18-H430).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12282-1 is reactive to OC90 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.445kDa
Background of Oc90
Predicted to enable calcium ion binding activity and structural molecule activity. Predicted to be involved in otolith mineralization. Predicted to be located in extracellular region. Predicted to be active in extracellular matrix.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12282-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human SH-SY5Y whole cell, rat brain tissue, mouse brain tissue, mouse testis tissue, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OC90 using anti-OC90 antibody (A12282-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OC90 antigen affinity purified polyclonal antibody (Catalog # A12282-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OC90 at approximately 52 kDa. The expected band size for OC90 is at 52 kDa.
Protein Target Info & Infographic
Gene/Protein Information For OC90 (Source: Uniprot.org, NCBI)
Gene Name
OC90
Full Name
Otoconin-90
Weight
52.445kDa
Superfamily
phospholipase A2 family
Alternative Names
Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17 Oc90|Ocn-, Ocn-95, P, PLA2L, Pla2ll, oc95|otoconin 90|otoconin-90|otoconin-95|phospholipase A2-like like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OC90, check out the OC90 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OC90: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OC90 Antibody Picoband® (A12282-1)
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