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Product Info Summary
| SKU: | A05327-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, ICC, WB |
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Product info
Product Name
Anti-NUP133 Antibody Picoband®
SKU/Catalog Number
A05327-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUP133 Antibody Picoband® catalog # A05327-2. Tested in ELISA, IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUP133 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05327-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUP133 recombinant protein (Position: Q228-I1156).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05327-2 is reactive to NUP133 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
129 kDa
Calculated molecular weight
129 kDa
Background of NUP133
Nuclear pore complex protein Nup133, or Nucleoporin Nup133, is a protein that in humans is encoded by the NUP133 gene. The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05327-2 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human A549 whole cell, human MCF-7 whole cell, rat C6 whole cell, rat NRK whole cell, mouse Neuro-2a whole cell, mouse NIH/3T3 whole cell
ICC/IF: A549 cell, C6 cell
IP: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NUP133 using anti-NUP133 antibody (A05327-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP133 antigen affinity purified polyclonal antibody (Catalog # A05327-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP133 at approximately 129 kDa. The expected band size for NUP133 is at 129 kDa.
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Figure 2. IF analysis of NUP133 using anti-NUP133 antibody (A05327-2) and anti-Beta Tubulin antibody (M01857-3).
NUP133 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP133 Antibody (A05327-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. IF analysis of NUP133 using anti-NUP133 antibody (A05327-2) and anti-Beta Tubulin antibody (M01857-3).
NUP133 was detected in immunocytochemical section of C6 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP133 Antibody (A05327-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Immunoprecipitating NUP133 in Hela whole cell lysate .
Western blot analysis of NUP133 using anti-NUP133 antibody (A05327-2).
Lane 1: Hela whole cell lysates (30ug)
Lane 2: Rabbit control IgG instead of anti-NUP133 antibody in Hela whole cell lysate.
Lane 3: anti-NUP133 antibody (2μg) + Hela whole cell lysate (500μg)
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NUP133 antigen affinity purified polyclonal antibody (A05327-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NUP133 at approximately 129 kDa. The expected band size for NUP133 is at 129 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NUP133 (Source: Uniprot.org, NCBI)
Gene Name
NUP133
Full Name
Nuclear pore complex protein Nup133
Weight
129 kDa
Superfamily
nucleoporin Nup133 family
Alternative Names
Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097 NUP133 GAMOS8, NPHS18, hNUP133 nucleoporin 133 nuclear pore complex protein Nup133|133 kDa nucleoporin|nucleoporin 133kD|nucleoporin 133kDa|nucleoporin Nup133
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUP133, check out the NUP133 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUP133: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUP133 Antibody Picoband® (A05327-2)
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