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Product Info Summary
| SKU: | A07995 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NUDT9 Antibody Picoband®
SKU/Catalog Number
A07995
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUDT9 Antibody Picoband® catalog # A07995. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUDT9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07995)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NUDT9 recombinant protein (Position: R4-L350). Human NUDT9 shares 89.3% and 89% amino acid (aa) sequence identity with mouse and rat NUDT9, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07995 is reactive to NUDT9 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39 kDa
Background of NUDT9
ADP-ribose pyrophosphatase, mitochondrial is an enzyme that in humans is encoded by the NUDT9 gene. The protein encoded by this gene belongs to the Nudix hydrolase family. Nudix boxes are found in a family of diverse enzymes that catalyze the hydrolysis of nucleoside diphosphate derivatives. This enzyme is an ADP-ribose pyrophosphatase that catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. It requires divalent metal ions and an intact Nudix motif for enzymatic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07995 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Raji whole cell, human A431 whole cell, human Hela whole cell, human U20S whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
ICC/IF: A549 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NUDT9 using anti-NUDT9 antibody (A07995).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT9 antigen affinity purified polyclonal antibody (Catalog # A07995) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT9 at approximately 39 kDa. The expected band size for NUDT9 is at 39 kDa.
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Figure 2. IF analysis of NUDT9 using anti-NUDT9 antibody (A07995).
NUDT9 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUDT9 Antibody (A07995) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A431 cells using anti-NUDT9 antibody (A07995).
Overlay histogram showing A431 cells stained with A07995 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUDT9 Antibody (A07995, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NUDT9 (Source: Uniprot.org, NCBI)
Gene Name
NUDT9
Full Name
ADP-ribose pyrophosphatase, mitochondrial
Weight
39 kDa
Superfamily
Nudix hydrolase family
Alternative Names
ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ADP-ribose pyrosphosphatase NUDT9; ADPR-PPase; EC 3.6.1.13; MGC3037; mitochondrial; nucleoside diphosphate linked moiety X-type motif 9; nudix (nucleoside diphosphate linked moiety X)-type motif 9; Nudix motif 9; NUDT10 NUDT9 NUDT10 nudix hydrolase 9 ADP-ribose pyrophosphatase, mitochondrial|ADP-ribose diphosphatase|ADP-ribose phosphohydrolase|ADP-ribose pyrosphosphatase NUDT9|ADPR-PPase|adenosine diphosphoribose pyrophosphatase|nucleoside diphosphate linked moiety X-type motif 9|nudix (nucleoside diphosphate linked moiety X)-type motif 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUDT9, check out the NUDT9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUDT9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUDT9 Antibody Picoband® (A07995)
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