Anti-NOD3 NLRC3 Antibody

Product Name

Anti-NOD3 NLRC3 Antibody

View all NLRC3 Antibodies

SKU/Catalog Number

A07242

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NOD3 NLRC3 Antibody (Catalog # A07242). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NOD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NOD3 NLRC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07242)

Host

Rabbit

Contents

NOD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3. The immunogen is located within amino acids 630 - 680 of NOD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TTYH1 antibody is human specific. Multiple isoforms of TTYH1 are known to exist.

Reactive Species

A07242 is reactive to NLRC3 in Human, Mouse, Rat

Applications

A07242 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

114658 MW

Background of NLRC3

NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.

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Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.

Gene/Protein Information For NLRC3 (Source: Uniprot.org, NCBI)

Alternative Names

CARD15-Like Protein; Caterpiller Protein 16.2; CLR16.2; Leucine Rich Repeat And CARD Domain Containing 3; NLR Family, CARD Domain Containing 3; NOD3; NOD-Like Receptor C3; Nucleotide-Binding Oligomerization Domain Protein 3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And CARD Domain Containing 3; Protein NLRC3 NLRC3 CLR16.2, NOD3 NLR family CARD domain containing 3 NLR family CARD domain-containing protein 3|CARD15-like protein|NACHT, LRR and CARD domains-containing protein 3|NOD-like receptor C3|caterpiller protein 16.2|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3

For more info on NLRC3, check out the NLRC3 Infographic

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In this infographic, you will see the following information for NLRC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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