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Product Info Summary
| SKU: | A02676-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NNMT Antibody Picoband®
View all Nicotinamide N-Methyltransferase/NNMT Antibodies
SKU/Catalog Number
A02676-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NNMT Antibody Picoband® catalog # A02676-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NNMT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02676-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NNMT recombinant protein (Position: K8-L264). Human NNMT shares 85.1% amino acid (aa) sequence identity with mouse NNMT.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02676-2 is reactive to NNMT in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
29.574kDa
Background of Nicotinamide N-Methyltransferase/NNMT
Nicotinamide N-methyltransferase (NNMT) is an enzyme that in humans is encoded by the NNMT gene. N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02676-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human A549 whole cell, human SIHA whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NNMT using anti-NNMT antibody (A02676-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NNMT antigen affinity purified polyclonal antibody (Catalog # A02676-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NNMT at approximately 28 kDa. The expected band size for NNMT is at 30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NNMT (Source: Uniprot.org, NCBI)
Gene Name
NNMT
Full Name
Nicotinamide N-methyltransferase
Weight
29.574kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
EC 2.1.1.1; Nicotinamide NMethyltransferase; Nicotinamide N-Methyltransferase; NNMT NNMT nicotinamide N-methyltransferase nicotinamide N-methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NNMT, check out the NNMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NNMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NNMT Antibody Picoband® (A02676-2)
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