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Product Info Summary
| SKU: | A16202 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NKX24 Antibody
SKU/Catalog Number
A16202
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NKX24 Antibody (Catalog # A16202). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NKX24 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16202)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NKX24.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16202 is reactive to NKX2-4 in Human
Reconstitution
Calculated molecular weight
36179 MW
Background of Nkx2-4
Probable transcription factor.
Rogers J., Nature 414:865-871(2001).
Venter J.C., Submitted (SEP-2005).
Harvey R.P., Mamm. Genome 11:466-468(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16202 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa and COLO205 cells, using NKX24 antibody A16202.
Protein Target Info & Infographic
Gene/Protein Information For NKX2-4 (Source: Uniprot.org, NCBI)
Gene Name
NKX2-4
Full Name
Homeobox protein Nkx-2.4
Weight
36179 MW
Superfamily
NK-2 homeobox family
Alternative Names
Homeobox protein Nkx-2.4;Homeobox protein NK-2 homolog D;NKX2-4;NKX2D;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NKX2-4, check out the NKX2-4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NKX2-4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NKX24 Antibody (A16202)
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No publications found for A16202
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-NKX24 Antibody
Question
I was wanting to use your anti-NKX24 antibody for WB for human hypothalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hypothalamus identification?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
You can see on the product datasheet, A16202 anti-NKX24 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human hypothalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this A16202 anti-NKX24 antibody reactive to the isotypes of NKX2-4?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
The immunogen of A16202 anti-NKX24 antibody is Synthesized peptide derived from internal of human NKX24. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-07
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hypothalamus using anti-NKX24 antibody A16202. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-12
Question
We are currently using anti-NKX24 antibody A16202 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
The anti-NKX24 antibody (A16202) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-26
Question
Can you help my question with product A16202, anti-NKX24 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16202 anti-NKX24 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-19
