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Product Info Summary
| SKU: | A00341-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NGF Antibody Picoband®
SKU/Catalog Number
A00341-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NGF Antibody Picoband® catalog # A00341-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00341-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NGF, which shares 91.3% amino acid (aa) sequence identity with both mouse and rat NGF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00341-1 is reactive to NGF in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
26959 MW
Background of beta-NGF
Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. It is mapped to 1p13.2. This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00341-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Positive Control
WB: human U-87MG whole cell, human 22RV1 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NGF using anti-NGF antibody (A00341-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human 22RV1 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27KD. The expected band size for NGF is at 27KD.
Protein Target Info & Infographic
Gene/Protein Information For NGF (Source: Uniprot.org, NCBI)
Gene Name
NGF
Full Name
Beta-nerve growth factor
Weight
26959 MW
Superfamily
NGF-beta family
Alternative Names
Beta-nerve growth factor; Beta-NGF; NGF; NGFB NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGF Antibody Picoband® (A00341-1)
Hello CJ!
A00341-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Olfactory ensheathing cells promote migration of Schwann cells by secreted nerve growth factor
Temporal Profile of Nerve Growth Factor Expression in the Partial Central Nervous System of the Yangtze Alligator Alligator sinensis (Reptilia,Crocodylia) During Early Postnatal Growth
Temporal Profile of Nerve Growth Factor Expression in the Partial Central Nervous System of the Yangtze Alligator Alligator sinensis (Reptilia,Crocodylia) During Early Postnatal Growth
Temporal Profile of Nerve Growth Factor Expression in the Partial Central Nervous System of the Yangtze Alligator Alligator sinensis (Reptilia,Crocodylia) During Early Postnatal Growth
Serum EGF and NGF levels of patients with brain injury and limb fracture
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