Anti-Neuropeptide S/NPS Antibody Picoband®

NPS antibody

Boster Bio Anti-Neuropeptide S/NPS Antibody Picoband® catalog # A01290-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01290-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Neuropeptide S/NPS Antibody Picoband®

View all NPS Antibodies

SKU/Catalog Number

A01290-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neuropeptide S/NPS Antibody Picoband® catalog # A01290-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neuropeptide S/NPS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01290-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Neuropeptide S, which shares 80% amino acid (aa) sequence identity with both mouse and rat Neuropeptide S.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01290-1 is reactive to NPS in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

19 kDa

Calculated molecular weight

10.103kDa

Background of NPS

Neuropeptide S (NPS) is a neuropeptide found in human and mammalian brain, mainly produced by neurons in the amygdala and between Barrington's nucleus and the locus coeruleus, although NPS-responsive neurons extend projections into many other brain areas. NPS binds specifically to a G protein-coupled receptor, NPSR. Animal studies show that NPS suppresses anxiety and appetite, induces wakefulness and hyperactivity, including hyper-sexuality, and plays a significant role in the extinction of conditioned fear.It has also been shown to significantly enhance dopamine activity in the mesolimbic pathway, and inhibits motility and increases permeability in neurocrine fashion acting through NO in the myenteric plexus in rats and humans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01290-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: rat brain tissue, mouse brain tissue

Validation Images & Assay Conditions

Gene/Protein Information For NPS (Source: Uniprot.org, NCBI)

Gene Name

NPS

Full Name

Neuropeptide S

Weight

10.103kDa

Alternative Names

Neuropeptide S; NPS NPS neuropeptide S neuropeptide S|prepro-neuropeptide S

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NPS, check out the NPS Infographic

NPS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NPS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Neuropeptide S/NPS Antibody Picoband®

Question

Would A01290-1 anti-Neuropeptide S/NPS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

You can see on the product datasheet, A01290-1 anti-Neuropeptide S/NPS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-07

Question

Do you have a BSA free version of anti-Neuropeptide S/NPS antibody A01290-1 available?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Neuropeptide S/NPS antibody A01290-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-28

Question

Will anti-Neuropeptide S/NPS antibody A01290-1 work on zebrafish WB with placenta?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

Our lab technicians have not validated anti-Neuropeptide S/NPS antibody A01290-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Neuropeptide S/NPS antibody A01290-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-22

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Neuropeptide S/NPS antibody A01290-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-01

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-01

Question

We are currently using anti-Neuropeptide S/NPS antibody A01290-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?

C. Bhatt

Verified customer

Asked: 2017-10-13

Answer

The anti-Neuropeptide S/NPS antibody (A01290-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-13

Order DetailsPrice
A01290-1

100μg

$370
A01290-1-10ug

10μg sample (liquid)

$99
A01290-1-Biotin

100 μg Biotin conjugated

$570
A01290-1-Cy3

100 μg Cy3 conjugated

$570
A01290-1-Dylight488

100 μg Dylight488 conjugated

$570
A01290-1-Dylight550

100 μg Dylight550 conjugated

$570
A01290-1-Dylight594

100 μg Dylight594 conjugated

$570
A01290-1-FITC

100 μg FITC conjugated

$570
A01290-1-HRP

100 μg HRP conjugated

$570
A01290-1-APC

100 μg APC conjugated

$670
A01290-1-PE

100 μg PE conjugated

$670
A01290-1-iFluor647

100 μg iFluor647 conjugated

$670
A01290-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A01290-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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