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Product Info Summary
| SKU: | A01290-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Neuropeptide S/NPS Antibody Picoband®
SKU/Catalog Number
A01290-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neuropeptide S/NPS Antibody Picoband® catalog # A01290-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuropeptide S/NPS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01290-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Neuropeptide S, which shares 80% amino acid (aa) sequence identity with both mouse and rat Neuropeptide S.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01290-1 is reactive to NPS in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
10.103kDa
Background of NPS
Neuropeptide S (NPS) is a neuropeptide found in human and mammalian brain, mainly produced by neurons in the amygdala and between Barrington's nucleus and the locus coeruleus, although NPS-responsive neurons extend projections into many other brain areas. NPS binds specifically to a G protein-coupled receptor, NPSR. Animal studies show that NPS suppresses anxiety and appetite, induces wakefulness and hyperactivity, including hyper-sexuality, and plays a significant role in the extinction of conditioned fear.It has also been shown to significantly enhance dopamine activity in the mesolimbic pathway, and inhibits motility and increases permeability in neurocrine fashion acting through NO in the myenteric plexus in rats and humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01290-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuropeptide S antigen affinity purified polyclonal antibody (Catalog # A01290-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuropeptide S at approximately 19KD. The expected band size for Neuropeptide S is at 10KD.
Protein Target Info & Infographic
Gene/Protein Information For NPS (Source: Uniprot.org, NCBI)
Gene Name
NPS
Full Name
Neuropeptide S
Weight
10.103kDa
Alternative Names
Neuropeptide S; NPS NPS neuropeptide S neuropeptide S|prepro-neuropeptide S
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPS, check out the NPS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuropeptide S/NPS Antibody Picoband® (A01290-1)
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No publications found for A01290-1
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Customer Q&As
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5 Customer Q&As for Anti-Neuropeptide S/NPS Antibody Picoband®
Question
Would A01290-1 anti-Neuropeptide S/NPS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, A01290-1 anti-Neuropeptide S/NPS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-07
Question
Do you have a BSA free version of anti-Neuropeptide S/NPS antibody A01290-1 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Neuropeptide S/NPS antibody A01290-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
Will anti-Neuropeptide S/NPS antibody A01290-1 work on zebrafish WB with placenta?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Our lab technicians have not validated anti-Neuropeptide S/NPS antibody A01290-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Neuropeptide S/NPS antibody A01290-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Neuropeptide S/NPS antibody A01290-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-01
Question
We are currently using anti-Neuropeptide S/NPS antibody A01290-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
C. Bhatt
Verified customer
Asked: 2017-10-13
Answer
The anti-Neuropeptide S/NPS antibody (A01290-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-13
