Click image to see more details
Product Info Summary
| SKU: | A03549 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NAGLU Antibody Picoband®
View all alpha-N-acetylglucosaminidase/NAGLU Antibodies
SKU/Catalog Number
A03549
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAGLU Antibody Picoband® catalog # A03549. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NAGLU Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03549)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NAGLU recombinant protein (Position: D24-D489).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03549 is reactive to NAGLU in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
82.266kDa
Background of alpha-N-acetylglucosaminidase/NAGLU
N-acetylglucosaminidase, alpha is a protein that in humans is encoded by the NAGLU gene. This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03549 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NAGLU using anti-NAGLU antibody (A03549).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAGLU antigen affinity purified polyclonal antibody (A03549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAGLU at approximately 82 kDa. The expected band size for NAGLU is at 82 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NAGLU (Source: Uniprot.org, NCBI)
Gene Name
NAGLU
Full Name
Alpha-N-acetylglucosaminidase
Weight
82.266kDa
Superfamily
glycosyl hydrolase 89 family
Alternative Names
alphaNacetylglucosaminidase; alpha-N-acetylglucosaminidase; EC 3.2.1.50; MPS3B; MPS-IIIB; N-acetyl-alpha-glucosaminidase; N-acetylglucosaminidase, alpha; NAG; NAGalpha-N-acetylglucosaminidase; NAGLU; UFHSD; UFHSD1 NAGLU CMT2V, MPS-IIIB, MPS3B, NAG, UFHSD N-acetyl-alpha-glucosaminidase alpha-N-acetylglucosaminidase|N-acetylglucosaminidase, alpha|testicular tissue protein Li 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAGLU, check out the NAGLU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAGLU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAGLU Antibody Picoband® (A03549)
Hello CJ!
No publications found for A03549
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NAGLU Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NAGLU Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
