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Product Info Summary
| SKU: | A07831-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NaDC-1/SLC13A2 Antibody Picoband®
SKU/Catalog Number
A07831-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NaDC-1/SLC13A2 Antibody Picoband® catalog # A07831-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NaDC-1/SLC13A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07831-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NaDC-1/SLC13A2 recombinant protein (Position: A152-M417).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07831-1 is reactive to SLC13A2 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
49229 MW
Background of SLC13A2
Solute carrier family 13 member 2 is a protein that is encoded in humans by the SLC13A2 gene. The protein encoded by this gene is a sodium-coupled citrate transporter that is regulated by the chaperone activity of cyclophilin b. The encoded protein may play a role in the formation of kidney stones.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07831-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Caco-2 whole cell, rat kidney tissue, rat PC-12 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NaDC-1/SLC13A2 using anti-NaDC-1/SLC13A2 antibody (A07831-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NaDC-1/SLC13A2 antigen affinity purified polyclonal antibody (Catalog # A07831-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NaDC-1/SLC13A2 at approximately 70 kDa. The expected band size for NaDC-1/SLC13A2 is at 64 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SLC13A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC13A2
Full Name
Solute carrier family 13 member 2
Weight
49229 MW
Superfamily
SLC13A/DASS transporter (TC 2.A.47) family
Alternative Names
Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27 SLC13A2 NADC1, NaCT, NaDC-1, SDCT1 solute carrier family 13 member 2 solute carrier family 13 member 2|Na(+)-coupled citrate transporter|Na(+)/dicarboxylate cotransporter 1|renal sodium/dicarboxylate cotransporter|solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC13A2, check out the SLC13A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC13A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NaDC-1/SLC13A2 Antibody Picoband® (A07831-1)
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