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Product Info Summary
| SKU: | A05438-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-N WASP/WASL Antibody Picoband®
SKU/Catalog Number
A05438-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-N WASP/WASL Antibody Picoband® catalog # A05438-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N WASP/WASL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05438-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05438-3 is reactive to WASL in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
39411 MW
Background of N-WASP
Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05438-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human U87 whole cell, human HELA whole cell, human CACO-2 whole cell, rat brain tissue, rat lung tissue, rat liver tissue, rat C6 whole cell, mouse brain tissue lysayes, mouse lung tissue, mouse liver tissue, mouse Neuro-2a whole cell
FCM: HELA cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of N WASP/WASL using anti-N WASP/WASL antibody (A05438-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human HELA whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse brain tissue lysayes,
Lane 10: mouse lung tissue lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N WASP/WASL antigen affinity purified polyclonal antibody (Catalog # A05438-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N WASP/WASL at approximately 70KD. The expected band size for N WASP/WASL is at 70KD.
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Figure 2. Flow Cytometry analysis of HELA cells using anti-N WASP/WASL antibody (A05438-3).
Overlay histogram showing HELA cells stained with A05438-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-N WASP/WASL Antibody (A05438-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For WASL (Source: Uniprot.org, NCBI)
Gene Name
WASL
Full Name
Neural Wiskott-Aldrich syndrome protein
Weight
39411 MW
Alternative Names
POU domain, class 4, transcription factor 1 WASL N-WASP, NWASP, WASPB WASP like actin nucleation promoting factor neural Wiskott-Aldrich syndrome protein|Wiskott-Aldrich syndrome like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WASL, check out the WASL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WASL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-N WASP/WASL Antibody Picoband® (A05438-3)
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