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Product Info Summary
| SKU: | A09646-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MYL6 Antibody Picoband®
SKU/Catalog Number
A09646-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MYL6 Antibody Picoband® catalog # A09646-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MYL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09646-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequencein the middle region of human MYL6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09646-1 is reactive to MYL6 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.93kDa
Background of MYL6
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09646-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human SiHa whole cell lysates, human Hela whole cell lysates, human THP-1 whole cell lysates
FCM: Hela cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MYL6 using anti-MYL6 antibody (A09646-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL6 antigen affinity purified polyclonal antibody (Catalog # A09646-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL6 at approximately 16 kDa. The expected band size for MYL6 is at 16 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-MYL6 antibody (A09646-1).
Overlay histogram showing Hela cells stained with A09646-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYL6 Antibody (A09646-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MYL6 (Source: Uniprot.org, NCBI)
Gene Name
MYL6
Full Name
Myosin light polypeptide 6
Weight
16.93kDa
Alternative Names
ESMLC; LC17; LC17A; LC17B; LC17-GI; LC17-NM; MLC1SM; MLC-3; MLC3NM; MLC3SM; Myosin light chain 3; Myosin light chain A3; Myosin light chain alkali 3; myosin light polypeptide 6,17 kDa myosin light chain; myosin, light chain 6, alkali, smooth muscle and non-muscle; myosin, light polypeptide 6, alkali, smooth muscle and non-muscle; Smooth muscle and nonmuscle myosin light chain alkali 6 MYL6 ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3NM, MLC3SM myosin light chain 6 myosin light polypeptide 6|17 kDa myosin light chain|myosin light chain A3|myosin light chain alkali 3|myosin, light chain 6, alkali, smooth muscle and non-muscle|myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYL6, check out the MYL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYL6 Antibody Picoband® (A09646-1)
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