Anti-MRPS22 Antibody

Product Name

Anti-MRPS22 Antibody

View all MRPS22 Antibodies

SKU/Catalog Number

A10300

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPS22 Antibody (Catalog # A10300). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPS22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10300)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPS22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10300 is reactive to MRPS22 in Human

Reconstitution

Calculated molecular weight

41280 MW

Background of MRPS22

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq.

Crisponi L., Nat. Genet. 27:159-166(2001).
Li Y., Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10300 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MRPS22 (Source: Uniprot.org, NCBI)

Alternative Names

28S ribosomal protein S22, mitochondrial;MRP-S22;S22mt;MRPS22;C3orf5, RPMS22;GK002; MRPS22 C3orf5, COXPD5, GIBT, GK002, MRP-S22, ODG7, RPMS22 mitochondrial ribosomal protein S22 28S ribosomal protein S22, mitochondrial|S22mt|mitochondrial small ribosomal subunit protein mS22

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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