Anti-MRGE MRGPRE Antibody

MRGPRE antibody

Boster Bio Anti-MRGE MRGPRE Antibody catalog # A17659. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A17659
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-MRGE MRGPRE Antibody

View all MRGPRE Antibodies

SKU/Catalog Number

A17659

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MRGE MRGPRE Antibody catalog # A17659. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRGE MRGPRE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17659)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human MRGE. at AA rangle: 140-220

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A17659 is reactive to MRGPRE in Human, Mouse, Rat

Applications

A17659 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

34286 MW

Background of MRGPRE

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MRGPRE (Source: Uniprot.org, NCBI)

Gene Name

MRGPRE

Full Name

Mas-related G-protein coupled receptor member E

Weight

34286 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

G protein-coupled receptor 167; GPR167; G-protein coupled receptor 167; mas-related G protein-coupled MRGE; MAS-related GPR, member E; mas-related G-protein coupled receptor member E; MRGE; MRGEMGC138408; MRGPRE MRGPRE GPR167, MGRF, MRGE MAS related GPR family member E mas-related G-protein coupled receptor member E|G-protein coupled receptor 167|MAS-related GPR, member E|Mas-related G protein-coupled receptor F|mas-related G protein-coupled MRGE

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MRGPRE, check out the MRGPRE Infographic

MRGPRE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRGPRE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MRGE MRGPRE Antibody

Question

My question regarding product A17659, anti-MRGE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Anderson

Verified customer

Asked: 2020-03-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17659 anti-MRGE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-10

Question

Does A17659 anti-MRGE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

As indicated on the product datasheet, A17659 anti-MRGE antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-03

Question

Does anti-MRGE antibody A17659 work for WB with ectocervix?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

According to the expression profile of ectocervix, MRGPRE is highly expressed in ectocervix. So, it is likely that anti-MRGE antibody A17659 will work for WB with ectocervix.

Boster Scientific Support

Answered: 2019-10-16

Question

I am interested in to test anti-MRGE antibody A17659 on human ectocervix for research purposes, then I may be interested in using anti-MRGE antibody A17659 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

The products we sell, including anti-MRGE antibody A17659, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-15

Question

I was wanting to use your anti-MRGE antibody for WB for human ectocervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human ectocervix identification?

R. Johnson

Verified customer

Asked: 2013-02-06

Answer

You can see on the product datasheet, A17659 anti-MRGE antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human ectocervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-06

Order DetailsPrice
A17659

100uL

$399

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A17659
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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