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Product Info Summary
| SKU: | M05470-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103
View all Kappa Light Chain Antibodies
SKU/Catalog Number
M05470-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (Catalog # M05470-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM103
Isotype
Rabbit IgG
Immunogen
Mouse IgG
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross-reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
Reactive Species
M05470-2 is reactive to Igkc in Mouse
Reconstitution
Calculated molecular weight
11934 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05470-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.005ug/mL-0.2ug/mL
WB: 0.1ug/mL-0.5ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western blot of nonreduced(-) and reduced(+) mouse IgG1 and IgG1 (20ng/lane), using 0.2ug/mL of clone RM103. This antibody reacted to nonreduced IgG1 kappa (~150 kDa), and slightly react to reduced kappa light chain (~25 kDa)
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Figure 2. ELISA result showing specificity
ELISA of mouse immunoglobulins shows RM103 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 3. ELISA result showing specificity
A titer ELISA of mouse IgG1 Kappa. The plate was coated with different amounts of mouse IgG1 Kappa. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For Igkc (Source: Uniprot.org, NCBI)
Gene Name
Igkc
Full Name
Immunoglobulin kappa constant
Weight
11934 MW
Alternative Names
Immunoglobulin kappa constant ;Ig kappa chain C region MOPC 21 ;Igkc ; IGKC HCAK1D, Km, IGKC immunoglobulin kappa constant immunoglobulin kappa (invariant region)|immunoglobulin kappa constant region|immunoglobulin kappa light chain (VJ)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Igkc, check out the Igkc Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Igkc: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 (M05470-2)
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