Anti-Mouse CD3E DyLight® 488 conjugated Antibody

Product Name

Anti-Mouse CD3E DyLight® 488 conjugated Antibody

View all CD3E Antibodies

SKU/Catalog Number

A02675-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Mouse CD3E DyLight® 488 conjugated Antibody catalog # A02675-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Mouse CD3E DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02675-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02675-Dyl488 is reactive to Cd3e in Mouse

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

23.147kDa

Background of CD3E

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02675-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cd3e (Source: Uniprot.org, NCBI)

Alternative Names

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; Cd3e CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e , epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell receptor complex, epsilon subunit of T3|T-cell surface T3/Leu-4 epsilon chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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