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Product Info Summary
| SKU: | A02011-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MKK6/MAP2K6 Antibody Picoband®
SKU/Catalog Number
A02011-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MKK6/MAP2K6 Antibody Picoband® catalog # A02011-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MKK6/MAP2K6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02011-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MKK6/MAP2K6 recombinant protein (Position: R31-H72).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02011-3 is reactive to MAP2K6 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
37492 MW
Background of MKK6/MEK6
MAP2K6 (Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene. It is located on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediatedsignal transduction pathway, this gene is involved in many cellular processes such as stress-induced cell cycle arrest, transcription activation and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02011-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat kidney tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K6 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K6
Full Name
Dual specificity mitogen-activated protein kinase kinase 6
Weight
37492 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 6;MAP kinase kinase 6;MAPKK 6;2.7.12.2;MAPK/ERK kinase 6;MEK 6;Stress-activated protein kinase kinase 3;SAPK kinase 3;SAPKK-3;SAPKK3;MAP2K6;MEK6, MKK6, PRKMK6, SKK3; MAP2K6 MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3 mitogen-activated protein kinase kinase 6 dual specificity mitogen-activated protein kinase kinase 6|MAPK/ERK kinase 6|MAPKK 6|MEK 6|SAPK kinase 3|protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)|stress-activated protein kinase kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K6, check out the MAP2K6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MKK6/MAP2K6 Antibody Picoband® (A02011-3)
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