Click image to see more details
Product Info Summary
| SKU: | RP1051 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Midkine/MDK Antibody Picoband®
SKU/Catalog Number
RP1051
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Midkine/MDK Antibody catalog # RP1051. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Midkine/MDK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1051)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Midkine recombinant protein (Position: V21-D143). Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1051 is reactive to MDK in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
15585 MW
Background of Midkine
Midkine (MK or MDK), also known as neurite growth-promoting factor 2 (NEGF2), is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta (PTPζ), low density lipoprotein receptor-related protein (LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1051 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human SH-SY5Y whole cell, human U20S whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MDK using anti-MDK antibody (RP1051).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK antigen affinity purified polyclonal antibody (Catalog # RP1051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDK at approximately 16 kDa. The expected band size for MDK is at 16 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MDK (Source: Uniprot.org, NCBI)
Gene Name
MDK
Full Name
Midkine
Weight
15585 MW
Superfamily
pleiotrophin family
Alternative Names
Midkine;MK;Amphiregulin-associated protein;ARAP;Midgestation and kidney protein;Neurite outgrowth-promoting factor 2;Neurite outgrowth-promoting protein;MDK;MK1, NEGF2; MDK ARAP, MK, NEGF2 midkine midkine|amphiregulin-associated protein|midgestation and kidney protein|neurite growth-promoting factor 2|neurite outgrowth-promoting factor 2|retinoic acid inducible factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDK, check out the MDK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Midkine/MDK Antibody Picoband® (RP1051)
Hello CJ!
RP1051 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo
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Customer Q&As
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4 Customer Q&As for Anti-Midkine/MDK Antibody Picoband®
Question
My question regarding product RP1051, anti-Midkine/MDK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Edwards
Verified customer
Asked: 2019-11-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1051 anti-Midkine/MDK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-13
Question
Is this RP1051 anti-Midkine/MDK antibody reactive to the isotypes of MDK?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The immunogen of RP1051 anti-Midkine/MDK antibody is E.coli-derived human Midkine recombinant protein (Position: V21-D143). Human Midkine shares 87% and 89% amino acid (aa) sequence identity with mouse and rat Midkine, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-16
Question
We are currently using anti-Midkine/MDK antibody RP1051 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-Midkine/MDK antibody (RP1051) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
I see that the anti-Midkine/MDK antibody RP1051 works with WB, what is the protocol used to produce the result images on the product page?
J. Taylor
Verified customer
Asked: 2018-08-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-02
