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Product Info Summary
| SKU: | A32230-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MESDC2/MESD Antibody Picoband®
SKU/Catalog Number
A32230-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MESDC2/MESD Antibody Picoband® catalog # A32230-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MESDC2/MESD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32230-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MESDC2/MESD recombinant protein (Position: D80-Q205).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A32230-1 is reactive to MESD in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
14377 MW
Background of MESD
Mesoderm development candidate genes 1 (MESDC1) and 2 (MESDC2) were identified from the mesoderm development (MESD) deletion interval located on mouse chromosome 7. MESD acts as a chaperone for low-density lipoprotein receptor (LDLR) proteins in the ER, and particularly associates with Wnt signaling pathway coreceptors LRP5 and LPR6. MESD is required for proper LRP5/6 folding and maturation to the cell surface, which plays a major role in Wnt signaling. The interaction between MESD and LRP5 is disrupted by a G171V mutation in LRP5 that is found in individuals with a familial phenotype characterized by high bone mass.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32230-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Jurkat whole cell, human A549 whole cell, human Hela whole cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MESDC2/MESD using anti-MESDC2/MESD antibody (A32230-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESDC2/MESD antigen affinity purified polyclonal antibody (Catalog # A32230-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESDC2/MESD at approximately 24 kDa. The expected band size for MESDC2/MESD is at 26 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-MESDC2/MESD antibody (A32230-1).
Overlay histogram showing K562 cells stained with A32230-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MESDC2/MESD Antibody (A32230-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MESD (Source: Uniprot.org, NCBI)
Gene Name
MESD
Full Name
LRP chaperone MESD
Weight
14377 MW
Superfamily
MESD family
Alternative Names
Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29 MESD BOCAC2, OI20, MESD mesoderm development LRP chaperone LRP chaperone MESD|LDLR chaperone MESD|mesoderm development LRP chaperone MESD|mesoderm development candidate 2|mesoderm development protein|renal carcinoma NY-REN-61
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MESD, check out the MESD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MESD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MESDC2/MESD Antibody Picoband® (A32230-1)
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