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Product Info Summary
| SKU: | A03449-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ME1 Antibody Picoband®
SKU/Catalog Number
A03449-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ME1 Antibody Picoband® catalog # A03449-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ME1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03449-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ME1 recombinant protein (Position: M1-Q572).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03449-3 is reactive to ME1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
64 kDa
Calculated molecular weight
31203 MW
Background of ME1
NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03449-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Caco-2 whole cell, human Hela whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue
ICC/IF: Caco-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ME1 using anti-ME1 antibody (A03449-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (Catalog # A03449-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.
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Figure 2. IF analysis of ME1 using anti-ME1 antibody (A03449-3).
ME1 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ME1 Antibody (A03449-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ME1 (Source: Uniprot.org, NCBI)
Gene Name
ME1
Full Name
NADP-dependent malic enzyme
Weight
31203 MW
Superfamily
malic enzymes family
Alternative Names
High affinity copper uptake protein 1; Copper transporter 1; hCTR1; Solute carrier family 31 member 1; SLC31A1; COPT1; CTR1 ME1 HUMNDME, MES malic enzyme 1 NADP-dependent malic enzyme|Malic enzyme, cytoplasmic|NADP-ME|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+))|malic enzyme 1, NADP(+)-dependent, cytosolic|malic enzyme 1, soluble|pyruvic-malic carboxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ME1, check out the ME1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ME1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ME1 Antibody Picoband® (A03449-3)
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