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Product Info Summary
| SKU: | A07226-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MCM9 Antibody Picoband®
SKU/Catalog Number
A07226-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM9 Antibody Picoband® catalog # A07226-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MCM9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07226-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MCM9 recombinant protein (Position: M1-L644). Human MCM9 shares 92.7% and 93% amino acid (aa) sequence identity with mouse and rat MCM9, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07226-1 is reactive to MCM9 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
127 kDa
Calculated molecular weight
125.814kDa
Background of Mcm9
The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07226-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human 293T whole cell lysates, human MCF-7 whole cell lysate
FCM: MCF-7 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MCM9 using anti-MCM9 antibody (A07226-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM9 antigen affinity purified polyclonal antibody (Catalog # A07226-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM9 at approximately 127 kDa. The expected band size for MCM9 is at 127 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-MCM9 antibody (A07226-1).
Overlay histogram showing MCF-7 cells stained with A07226-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM9 Antibody (A07226-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MCM9 (Source: Uniprot.org, NCBI)
Gene Name
MCM9
Full Name
DNA helicase MCM9
Weight
125.814kDa
Superfamily
MCM family
Alternative Names
C6orf61; chromosome 6 open reading frame 61; dJ329L24.1; dJ329L24.3; DNA replication licensing factor MCM9; FLJ13942; FLJ20170; FLJ56845; hMCM9; MCMDC1; MGC35304; minichromosome maintenance complex component 9; Mini-chromosome maintenance deficient 9; minichromosome maintenance deficient domain containing 1; Mini-chromosome maintenance deficient domain-containing protein 1 Mcm9|9030408O17Rik, Gm235, Mcmdc1|minichromosome maintenance 9 homologous recombination repair factor|DNA helicase MCM9|DNA replication licensing factor MCM9|mini-chromosome maintenance deficient 9|mini-chromosome maintenance deficient domain-containing protein 1|minichromosome maintenance complex component 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCM9, check out the MCM9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCM9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM9 Antibody Picoband® (A07226-1)
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