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Product Info Summary
| SKU: | PA2042 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MBD4 Antibody Picoband®
SKU/Catalog Number
PA2042
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MBD4 Antibody catalog # PA2042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2042)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2042 is reactive to MBD4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
66051 MW
Background of MBD4
MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2042 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Kidney Tissue, A549 Cell, HELA Cell, MCF-7 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MBD4 antibody, PA2042, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD
(162aa tag+ Q400-S580)
Lane 1: Recombinant Human MBD4 Protein 10ng
Lane 2: Recombinant Human MBD4 Protein 5ng
Lane 3: Recombinant Human MBD4 Protein 2.5ng
Click image to see more details
Anti-MBD4 antibody, PA2042, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: MCF-7 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For MBD4 (Source: Uniprot.org, NCBI)
Gene Name
MBD4
Full Name
Methyl-CpG-binding domain protein 4
Weight
66051 MW
Alternative Names
Methyl-CpG-binding domain protein 4;3.2.2.-;Methyl-CpG-binding endonuclease 1;Methyl-CpG-binding protein MBD4;Mismatch-specific DNA N-glycosylase;MBD4;MED1; MBD4 MED1 methyl-CpG binding domain 4, DNA glycosylase methyl-CpG-binding domain protein 4|3,N(4)-ethenocytosine glycosylase|G/5-fluorouracil mismatch glycosylase with biphasic kinetics|G/T mismatch glycosylase|G/U mismatch glycosylase|methyl-CpG binding domain protein 4|methyl-CpG-binding endonuclease 1|methyl-CpG-binding protein MBD4|mismatch-specific DNA N-glycosylase|putative methyl-CpG binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD4, check out the MBD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD4 Antibody Picoband® (PA2042)
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No publications found for PA2042
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-MBD4 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MBD4 antibody (PA2042)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-MBD4 antibody (PA2042). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-MBD4 antibody PA2042 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The anti-MBD4 antibody (PA2042) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-13
Question
Does PA2042 anti-MBD4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Dhar
Verified customer
Asked: 2014-08-20
Answer
You can see on the product datasheet, PA2042 anti-MBD4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-20
Question
Can you help my question with product PA2042, anti-MBD4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Dhar
Verified customer
Asked: 2013-10-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2042 anti-MBD4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-10-01
Question
I would like to test anti-MBD4 antibody PA2042 on human fetal brain for research purposes, then I may be interested in using anti-MBD4 antibody PA2042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Gonzalez
Verified customer
Asked: 2013-07-17
Answer
The products we sell, including anti-MBD4 antibody PA2042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-17
