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Product Info Summary
| SKU: | A10839 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MAN2C1 Antibody Picoband®
SKU/Catalog Number
A10839
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAN2C1 Antibody Picoband® catalog # A10839. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MAN2C1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10839)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MAN2C1 recombinant protein (Position: A176-Q1027). Human MAN2C1 shares 90.5% and 87.3% amino acid (aa) sequence identity with mouse and rat MAN2C1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10839 is reactive to MAN2C1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
115.835kDa
Background of MAN2C1
MAN2C1 belongs to the glycosyl hydrolase 38 family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10839 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human HepG2 whole cell, human THP-1 whole cell, human RT4 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
ICC/IF: U2OS cell
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAN2C1 using anti-MAN2C1 antibody (A10839).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN2C1 antigen affinity purified polyclonal antibody (Catalog # A10839) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN2C1 at approximately 100 kDa. The expected band size for MAN2C1 is at 116 kDa.
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Figure 2. IF analysis of MAN2C1 using anti-MAN2C1 antibody (A10839) and anti-Beta Tubulin antibody (M01857-3).
MAN2C1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MAN2C1 Antibody (A10839) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of JK cells using anti-MAN2C1 antibody (A10839).
Overlay histogram showing JK cells stained with A10839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAN2C1 Antibody (A10839, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MAN2C1 (Source: Uniprot.org, NCBI)
Gene Name
MAN2C1
Full Name
Alpha-mannosidase 2C1
Weight
115.835kDa
Superfamily
glycosyl hydrolase 38 family
Alternative Names
Alpha-mannosidase 2C1 MAN2C1 MAN6A8, MANA, MANA1 mannosidase alpha class 2C member 1 alpha-mannosidase 2C1|alpha mannosidase 6A8B|alpha-D-mannoside mannohydrolase|mannosidase, alpha 6A8|mannosidase, alpha A, cytoplasmic|testicular tissue protein Li 115
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAN2C1, check out the MAN2C1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAN2C1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAN2C1 Antibody Picoband® (A10839)
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