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Product Info Summary
| SKU: | A09301-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LYRM4 Antibody Picoband®
SKU/Catalog Number
A09301-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LYRM4 Antibody Picoband® catalog # A09301-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LYRM4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09301-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LYRM4 recombinant protein (Position: M1-T91). Human LYRM4 shares 89% amino acid (aa) sequence identity with mouse LYRM4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09301-1 is reactive to LYRM4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
10.758kDa
Background of LYRM4
The protein encoded by this gene is found in both mitochondria and the nucleus, where it binds cysteine desulfurase and helps free inorganic sulfur for Fe/S clusters. Disruption of this gene negatively impacts mitochondrial and cytosolic iron homeostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09301-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LYRM4 using anti-LYRM4 antibody (A09301-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRM4 antigen affinity purified polyclonal antibody (Catalog # A09301-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRM4 at approximately 15 kDa. The expected band size for LYRM4 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LYRM4 (Source: Uniprot.org, NCBI)
Gene Name
LYRM4
Full Name
LYR motif-containing protein 4
Weight
10.758kDa
Superfamily
complex I LYR family
Alternative Names
C6orf149LYR motif-containing protein 4; CGI-203; chromosome 6 open reading frame 149; ISD11homolog of yeast Isd11; LYR motif containing 4; mitochondrial matrix Nfs1 interacting protein LYRM4 C6orf149, CGI-203, COXPD19, ISD11 LYR motif containing 4 LYR motif-containing protein 4|homolog of yeast Isd11|mitochondrial matrix Nfs1 interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LYRM4, check out the LYRM4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LYRM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LYRM4 Antibody Picoband® (A09301-1)
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