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Product Info Summary
| SKU: | A02742-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-LSR Antibody Picoband®
View all Lipolysis Stimulated Lipoprotein Receptor Antibodies
SKU/Catalog Number
A02742-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LSR Antibody Picoband® catalog # A02742-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LSR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02742-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LSR recombinant protein (Position: K16-E633).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02742-2 is reactive to LSR in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
12252 MW
Background of Lipolysis Stimulated Lipoprotein Receptor
Lipolysis-stimulated lipoprotein receptor is a protein that in humans is encoded by the LSR gene. LSR has a probable role in the clearance of triglyceride-rich lipoprotein from blood. It binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02742-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, human A431 whole cell, human Hacat whole cell, rat liver tissue, mouse liver tissue
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LSR using anti-LSR antibody (A02742).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSR antigen affinity purified polyclonal antibody (Catalog # A02742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSR at approximately 65 kDa. The expected band size for LSR is at 65 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-LSR antibody (A02742-2).
Overlay histogram showing MCF-7 cells stained with A02742-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LSR Antibody (A02742-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For LSR (Source: Uniprot.org, NCBI)
Gene Name
LSR
Full Name
Lipolysis-stimulated lipoprotein receptor
Weight
12252 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Platelet basic protein; PBP; C-X-C motif chemokine 7; Leukocyte-derived growth factor; LDGF; Macrophage-derived growth factor; MDGF; Small-inducible cytokine B7; PPBP; CTAP3; CXCL7; SCYB7; TGB1; THBGB1; NAP-2 LSR ILDR3, LISCH7 lipolysis stimulated lipoprotein receptor lipolysis-stimulated lipoprotein receptor|LISCH protein|angulin-1|immunoglobulin-like domain containing receptor 3|lipolysis-stimulated remnant|liver-specific bHLH-Zip transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LSR, check out the LSR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LSR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LSR Antibody Picoband® (A02742-2)
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