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Product Info Summary
| SKU: | A11132-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LSM11 Antibody Picoband®
SKU/Catalog Number
A11132-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LSM11 Antibody Picoband® catalog # A11132-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LSM11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11132-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LSM11 recombinant protein (Position: A17-Q360). Human LSM11 shares 88.4% amino acid (aa) sequence identity with mouse LSM11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11132-1 is reactive to LSM11 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
39.907kDa
Background of Lsm11
LSM11 is a component of the U7 snRNP complex that is involved in the histone 3'-end pre-mRNA processing .Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cellcycle progression from G1 to S phases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11132-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat testis tissue, rat skin tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LSM11 using anti-LSM11 antibody (A11132-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat skin tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSM11 antigen affinity purified polyclonal antibody (Catalog # A11132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSM11 at approximately 68 kDa. The expected band size for LSM11 is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LSM11 (Source: Uniprot.org, NCBI)
Gene Name
LSM11
Full Name
U7 snRNA-associated Sm-like protein LSm11
Weight
39.907kDa
Superfamily
snRNP Sm proteins family
Alternative Names
2210404M20Rik; FLJ38273; LSM11 homolog, U7 small nuclear RNA associated (S. cerevisiae); LSM11 homolog, U7 small nuclear RNA associated; LSM11, U7 small nuclear RNA associated; U7 snRNA-associated Sm-like protein LSm11 Lsm11|2210404M20Rik, AV079530|U7 snRNP-specific Sm-like protein LSM11|U7 snRNA-associated Sm-like protein LSm11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LSM11, check out the LSM11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LSM11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LSM11 Antibody Picoband® (A11132-1)
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