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Product Info Summary
| SKU: | A16326-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LRRC40 Antibody Picoband®
SKU/Catalog Number
A16326-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRRC40 Antibody Picoband® catalog # A16326-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LRRC40 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16326-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LRRC40 recombinant protein (Position: R21-D598). Human LRRC40 shares 84.1% amino acid (aa) sequence identity with mouse LRRC40.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16326-1 is reactive to LRRC40 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
68.25kDa
Background of LRRC40
Leucine rich repeat containing 40 (LRRC40) is a protein that in humans is encoded by the LRRC40 gene. The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic α/β horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. The primary function of these motifs is to provide a versatile structural framework to mediate the formation of protein-protein interactions. LRRs are present in a variety of proteins with diverse structure and function, including innate immunity and nervous system development. Several human diseases are associated with mutations in genes encoding LRR-containing proteins. LRRC40 (leucine rich repeat containing 40) is a 602 amino acid protein that contains 20 LRR (leucine-rich) repeats.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16326-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human RT4 whole cell, human K562 whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LRRC40 using anti-LRRC40 antibody (A16326-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC40 antigen affinity purified polyclonal antibody (Catalog # A16326-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC40 at approximately 72 kDa. The expected band size for LRRC40 is at 68 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LRRC40 (Source: Uniprot.org, NCBI)
Gene Name
LRRC40
Full Name
Leucine-rich repeat-containing protein 40
Weight
68.25kDa
Alternative Names
dJ677H15.1; FLJ20331; leucine rich repeat containing 40; leucine-rich repeat-containing protein 40 LRRC40 dJ677H15.1 leucine rich repeat containing 40 leucine-rich repeat-containing protein 40|testicular tissue protein Li 109
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRC40, check out the LRRC40 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRC40: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRC40 Antibody Picoband® (A16326-1)
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