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Product Info Summary
| SKU: | A19664 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LRRC30 Antibody Picoband®
SKU/Catalog Number
A19664
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRRC30 Antibody Picoband® catalog # A19664. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LRRC30 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19664)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LRRC30 recombinant protein (Position: H49-K298). Human LRRC30 shares 91.6% amino acid (aa) sequence identity with mouse LRRC30.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A19664 is reactive to LRRC30 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
33.931kDa
Background of Lrrc30
LRRC30 (Leucine Rich Repeat Containing 30) is a Protein Coding gene. An important paralog of this gene is SHOC2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A19664 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LRRC30 using anti-LRRC30 antibody (A19664).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC30 antigen affinity purified polyclonal antibody (Catalog # A19664) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC30 at approximately 45 kDa. The expected band size for LRRC30 is at 34 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LRRC30 (Source: Uniprot.org, NCBI)
Gene Name
LRRC30
Full Name
Leucine-rich repeat-containing protein 30
Weight
33.931kDa
Alternative Names
Leucine Rich Repeat Containing 30; Leucine-Rich Repeat-Containing Protein 30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRC30, check out the LRRC30 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRC30: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRC30 Antibody Picoband® (A19664)
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