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Product Info Summary
| SKU: | A16307-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-LMNTD1 Antibody Picoband®
SKU/Catalog Number
A16307-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LMNTD1 Antibody Picoband® catalog # A16307-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LMNTD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16307-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LMNTD1 recombinant protein (Position: Y48-E349). Human LMNTD1 shares 59.4% amino acid (aa) sequence identity with mouse LMNTD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16307-1 is reactive to LMNTD1 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
45.937kDa
Background of Lmntd1
The intermediate filament tail domain-containing protein (IFLTD1) was initially identified as a candidate gene for pulmonary adenoma susceptibility 1 gene in mice. Transcripts of the gene were only detected in mouse lung tissue from strains carrying the Pas1-susceptible allele. Expression of different alleles of this gene in lung cancer cell lines resulted in different levels of colony formation in in vitro colony formation assays, suggesting that allelic variants of this gene can modulate growth of human cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16307-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human RT4 whole cell, human K562 whole cell, human HepG2 whole cell, rat C6 whole cell
FCM: RT4 cell, SH-SY5Y cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IFTLD1/LMNTD1 using anti-IFTLD1/LMNTD1 antibody (A16307-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFTLD1/LMNTD1 antigen affinity purified polyclonal antibody (Catalog # A16307-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFTLD1/LMNTD1 at approximately 43 kDa. The expected band size for IFTLD1/LMNTD1 is at 43 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-IFTLD1/LMNTD1 antibody (A16307-1).
Overlay histogram showing RT4 cells stained with A16307-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFTLD1/LMNTD1 Antibody (A16307-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 3. Flow Cytometry analysis of SH-SY5Y cells using anti-IFTLD1/LMNTD1 antibody (A16307-1).
Overlay histogram showing SH-SY5Y cells stained with A16307-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFTLD1/LMNTD1 Antibody (A16307-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LMNTD1 (Source: Uniprot.org, NCBI)
Gene Name
LMNTD1
Full Name
Lamin tail domain-containing protein 1
Weight
45.937kDa
Superfamily
intermediate filament family
Alternative Names
Lamin tail domain-containing protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNTD1, check out the LMNTD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNTD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LMNTD1 Antibody Picoband® (A16307-1)
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