Anti-LINGO2 Antibody Picoband™

Product Name

Anti-LINGO2 Antibody Picoband™

View all LINGO-2 Antibodies

SKU/Catalog Number

A12658-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LINGO2 Antibody Picoband™ catalog # A12658-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LINGO2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12658-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human LINGO2 recombinant protein (Position: C28-R513). Human LINGO2 shares 97.9% amino acid (aa) sequence identity with mouse LINGO2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12658-1 is reactive to LINGO2 in Human, Mouse, Rat

Applications

A12658-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

Background of LINGO-2

Leucine rich repeat and Ig domain containing 2 is a protein that in humans is encoded by the LINGO2 gene. LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also LRRN6C and LERN3), is a member of the LRR transmembrane (TM) protein family. LINGO-2 is thought to function outside the CNS with little involvement by p75/NgR1. The human LINGO-2 precursor is a 606 amino acid (aa) type I TM protein that contains a large extracellular domain and a short cytoplasmic tail. The human LINGO-2 precursor is 99% and 98% aa identical to dog and mouse LINGO-2, respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of LINGO2 using anti-LINGO2 antibody (A12658-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LINGO2 antigen affinity purified polyclonal antibody (Catalog # A12658-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LINGO2 at approximately 68 kDa. The expected band size for LINGO2 is at 68 kDa.

Gene/Protein Information For LINGO2 (Source: Uniprot.org, NCBI)

Alternative Names

LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C LINGO2 LERN3, LRRN6C leucine rich repeat and Ig domain containing 2 leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2|leucine rich repeat neuronal 6C|leucine-rich repeat neuronal protein 3|leucine-rich repeat neuronal protein 6C

For more info on LINGO2, check out the LINGO2 Infographic

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In this infographic, you will see the following information for LINGO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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