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Product Info Summary
| SKU: | A12658-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LINGO2 Antibody Picoband®
SKU/Catalog Number
A12658-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LINGO2 Antibody Picoband® catalog # A12658-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LINGO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12658-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LINGO2 recombinant protein (Position: C28-R513). Human LINGO2 shares 97.9% amino acid (aa) sequence identity with mouse LINGO2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12658-1 is reactive to LINGO2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.066kDa
Background of LINGO-2
Leucine rich repeat and Ig domain containing 2 is a protein that in humans is encoded by the LINGO2 gene. LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also LRRN6C and LERN3), is a member of the LRR transmembrane (TM) protein family. LINGO-2 is thought to function outside the CNS with little involvement by p75/NgR1. The human LINGO-2 precursor is a 606 amino acid (aa) type I TM protein that contains a large extracellular domain and a short cytoplasmic tail. The human LINGO-2 precursor is 99% and 98% aa identical to dog and mouse LINGO-2, respectively.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12658-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human U251 whole cell, human Hela whole cell, human PC-3 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LINGO2 using anti-LINGO2 antibody (A12658-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LINGO2 antigen affinity purified polyclonal antibody (Catalog # A12658-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LINGO2 at approximately 68 kDa. The expected band size for LINGO2 is at 68 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LINGO2 (Source: Uniprot.org, NCBI)
Gene Name
LINGO2
Full Name
Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2
Weight
68.066kDa
Alternative Names
LERN3; LERN3FLJ31810; leucine rich repeat and Ig domain containing 2; leucine-rich repeat and immunoglobulin-like domain-containing nogoreceptor-interacting protein 2; Leucine-rich repeat neuronal protein 3; Leucine-rich repeat neuronal protein 6C; LINGO2; LINGO-2; LRRN6C; LRRN6Cleucine rich repeat neuronal 6C LINGO2 LERN3, LRRN6C leucine rich repeat and Ig domain containing 2 leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2|leucine rich repeat neuronal 6C|leucine-rich repeat neuronal protein 3|leucine-rich repeat neuronal protein 6C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LINGO2, check out the LINGO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LINGO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LINGO2 Antibody Picoband® (A12658-1)
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