Anti-LCMT2 Antibody Picoband™

Product Name

Anti-LCMT2 Antibody Picoband™

View all LCMT2 Antibodies

SKU/Catalog Number

A14797-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-LCMT2 Antibody Picoband™ catalog # A14797-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LCMT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14797-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human LCMT2 recombinant protein (Position: A29-N595).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A14797-1 is reactive to LCMT2 in Human, Mouse, Rat

Applications

A14797-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

76 kDa

Calculated molecular weight

52588 MW

Background of LCMT2

Leucine carboxyl methyltransferase 2 is an enzyme that in humans is encoded by the LCMT2 gene. The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LCMT2 using anti-LCMT2 antibody (A14797-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCMT2 antigen affinity purified polyclonal antibody (Catalog # A14797-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCMT2 at approximately 76 kDa. The expected band size for LCMT2 is at 76 kDa.

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Figure 2. IF analysis of LCMT2 using anti-LCMT2 antibody (A14797-1).
LCMT2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LCMT2 Antibody (A14797-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For LCMT2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.-; FLJ51321; KIAA0547tRNA-Wybutosine-synthesizing protein 4; leucine carboxyl methyltransferase 2; MGC9534; p21WAF1/CIP1 promoter-interacting protein; PPM2; tRNA wybutosine-synthesizing protein 4 homolog; tRNA yW-synthesizing protein 4 homolog; TYW4tRNA-yW synthesizing protein 4 LCMT2 PPM2, TYW4 leucine carboxyl methyltransferase 2 tRNA wybutosine-synthesizing protein 4|p21WAF1/CIP1 promoter-interacting protein|tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase|tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase|tRNA-Wybutosine-synthesizing protein 4|tRNA-yW synthesizing protein 4

For more info on LCMT2, check out the LCMT2 Infographic

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In this infographic, you will see the following information for LCMT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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