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Product Info Summary
| SKU: | M01732 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC |
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Product info
Product Name
Anti-Ku (p70/p80) (Nuclear Marker) XRCC6 Monoclonal Antibody
View all XRCC6 (p70) & XRCC5 (p80) Antibodies
SKU/Catalog Number
M01732
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Ku (p70/p80) (Nuclear Marker) XRCC6 Monoclonal Antibody (Catalog # M01732). Tested in Flow Cytometry, IF, ICC applications. This antibody reacts with Human, Primate.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Ku (p70/p80) (Nuclear Marker) XRCC6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01732)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: KU729
Isotype
IgG1, kappa
Immunogen
Nuclear extract of human HL-60 cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01732 is reactive to XRCC6 (p70) & XRCC5 (p80) in Human, Primate
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
69843 MW
Background of XRCC6 (p70) & XRCC5 (p80)
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01732 is guaranteed for Flow Cytometry, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT),Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Immunofluorescent analysis of PFA-fixed HeLa cells. Anti-Ku Mouse Monoclonal Antibody (KU729) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin (red).
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Flow Cytometry of human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Ku Mouse Monoclonal Antibody (KU729).
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Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Ku Mouse Monoclonal Antibody (KU729).
Protein Target Info & Infographic
Gene/Protein Information For XRCC6 (p70) & XRCC5 (p80) (Source: Uniprot.org, NCBI)
Gene Name
XRCC6 (p70) & XRCC5 (p80)
Full Name
Weight
69843 MW
Alternative Names
X-ray repair cross-complementing protein 6;3.6.4.-;4.2.99.-;5'-deoxyribose-5-phosphate lyase Ku70;5'-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit;CTC box-binding factor 75 kDa subunit;CTC75;CTCBF;DNA repair protein XRCC6;Lupus Ku autoantigen protein p70;Ku70;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 6;XRCC6;G22P1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC6 (p70) & XRCC5 (p80), check out the XRCC6 (p70) & XRCC5 (p80) Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC6 (p70) & XRCC5 (p80): database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ku (p70/p80) (Nuclear Marker) XRCC6 Monoclonal Antibody (M01732)
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No publications found for M01732
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Ku (p70/p80) (Nuclear Marker) XRCC6 Monoclonal Antibody
Question
Can you help my question with product M01732, anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01732 anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody (M01732)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do provide the blocking peptide for product anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody (M01732). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-12
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody M01732. Please let me know if you require anything else.
T. Parker
Verified customer
Asked: 2017-09-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-08
Question
My question regards to test anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody M01732 on human cervix carcinoma for research purposes, then I may be interested in using anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody M01732 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Wu
Verified customer
Asked: 2016-10-12
Answer
The products we sell, including anti-Ku (p70/p80) (Nuclear Marker) Monoclonal antibody M01732, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-10-12
