Anti-KAPCB PRKACB Antibody

PKA C beta antibody

Boster Bio Anti-KAPCB PRKACB Antibody (Catalog # A05366). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05366
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-KAPCB PRKACB Antibody

View all PKA C beta Antibodies

SKU/Catalog Number

A05366

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-KAPCB PRKACB Antibody (Catalog # A05366). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KAPCB PRKACB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05366)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human KAPCB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05366 is reactive to PRKACB in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

40623 MW

Background of PKA C beta

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.

Beebe S.J., Mol. Endocrinol. 4:465-475(1990).
Gregory S.G., Nature 441:315-321(2006).
Wu K.-J., Oncogene 21:7872-7882(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05366 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For PRKACB (Source: Uniprot.org, NCBI)

Gene Name

PRKACB

Full Name

cAMP-dependent protein kinase catalytic subunit beta

Weight

40623 MW

Superfamily

protein kinase superfamily

Alternative Names

cAMP-dependent protein kinase catalytic subunit beta;PKA C-beta;2.7.11.11;PRKACB; PRKACB CAFD2, PKA C-beta, PKACB protein kinase cAMP-activated catalytic subunit beta cAMP-dependent protein kinase catalytic subunit beta|protein kinase A catalytic subunit beta|protein kinase, cAMP-dependent, beta catalytic subunit|protein kinase, cAMP-dependent, catalytic, beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKACB, check out the PRKACB Infographic

PRKACB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKACB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-KAPCB PRKACB Antibody

Question

Is this A05366 anti-KAPCB antibody reactive to the isotypes of PRKACB?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The immunogen of A05366 anti-KAPCB antibody is Synthesized peptide derived from internal of human KAPCB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-06

Question

Will A05366 anti-KAPCB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

You can see on the product datasheet, A05366 anti-KAPCB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-03

Question

We are currently using anti-KAPCB antibody A05366 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

The anti-KAPCB antibody (A05366) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-11

Question

I was wanting to use your anti-KAPCB antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?

C. Krishna

Verified customer

Asked: 2016-09-13

Answer

It shows on the product datasheet, A05366 anti-KAPCB antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-09-13

Order DetailsPrice
A05366-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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