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Product Info Summary
| SKU: | A14937 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Interferon-inducible GTPase 5 IRGC Antibody
SKU/Catalog Number
A14937
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Interferon-inducible GTPase 5 IRGC Antibody (Catalog # A14937). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IRGC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Interferon-inducible GTPase 5 IRGC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14937)
Host
Rabbit
Contents
IRGC Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC. The immunogen is located within the last 50 amino acids of IRGC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
Reactive Species
A14937 is reactive to IRGC in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
50288 MW
Background of IRGC
Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14937 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.
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Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IRGC (Source: Uniprot.org, NCBI)
Gene Name
IRGC
Full Name
Interferon-inducible GTPase 5
Weight
50288 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
IFGGE, IRGC1, Iigp5, CINEMA, R30953_1, IIGP5, Interferon-inducible GTPase 5, Immunity-related GTPase cinema 1 IRGC CINEMA, IFGGE1, Iigp5, R30953_1, IRGC immunity related GTPase cinema interferon-inducible GTPase 5|immunity-related GTPase cinema 1|immunity-related GTPase family, cinema 1|interferon-gamma-inducible GTPase IFGGE protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRGC, check out the IRGC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRGC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon-inducible GTPase 5 IRGC Antibody (A14937)
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