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Product Info Summary
| SKU: | M06777 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Inhibin beta A Rabbit Monoclonal Antibody
SKU/Catalog Number
M06777
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Inhibin beta A Rabbit Monoclonal Antibody catalog # M06777. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Inhibin beta A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06777)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24I91
Isotype
IgG
Immunogen
A synthesized peptide derived from human Inhibin beta A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06777 is reactive to INHBA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
10685 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06777 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human U2OS whole cell, human PC-3 whole cell, human Hacat whole cell, human U251 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Inhibin beta A/INHBA using anti-Inhibin beta A/INHBA antibody (M06777).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Inhibin beta A/INHBA antigen affinity purified monoclonal antibody (M06777) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Inhibin beta A/INHBA at approximately 43 kDa. The expected band size for Inhibin beta A/INHBA is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For INHBA (Source: Uniprot.org, NCBI)
Gene Name
INHBA
Full Name
Inhibin beta A chain
Weight
10685 MW
Superfamily
TGF-beta family
Alternative Names
Small ubiquitin-related modifier 4;SUMO-4;Small ubiquitin-like protein 4;SUMO4;SMT3H4; INHBA EDF, FRP inhibin subunit beta A inhibin beta A chain|FSH-releasing protein|Inhibin, beta-1|activin beta-A chain|erythroid differentiation factor|erythroid differentiation protein|follicle-stimulating hormone-releasing protein|inhibin beta A subunit|inhibin, beta A (activin A, activin AB alpha polypeptide)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INHBA, check out the INHBA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INHBA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Inhibin beta A Rabbit Monoclonal Antibody (M06777)
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