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Product Info Summary
| SKU: | PB9521 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL7 Antibody Picoband®
SKU/Catalog Number
PB9521
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL7 Antibody Picoband® catalog # PB9521. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9521)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL7 recombinant protein (Position: D26-H177). Human IL7 shares 56.4% and 57% amino acid (aa) sequence identity with mouse and rat IL7, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9521 is reactive to IL7 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
20187 MW
Background of IL-7
IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9521 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: K562 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PB9521).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: K562 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9521) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 75 kDa. The expected band size for IL7 is at 20 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IL7 (Source: Uniprot.org, NCBI)
Gene Name
IL7
Full Name
Interleukin-7
Weight
20187 MW
Superfamily
IL-7/IL-9 family
Alternative Names
Interleukin-7;IL-7;IL7; IL7 IL-7 interleukin 7 interleukin-7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL7, check out the IL7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL7 Antibody Picoband® (PB9521)
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Customer Q&As
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5 Customer Q&As for Anti-IL7 Antibody Picoband®
Question
We were satisfied with the WB result of your anti-IL7 antibody. However we have seen positive staining in pancreas secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
From what I have seen in literature, pancreas does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-05-01
Question
My lab would like using your anti-IL7 antibody for positive regulation of cell population proliferation studies. Has this antibody been tested with western blotting on k562 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you for your inquiry. This PB9521 anti-IL7 antibody is tested on k562 whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-13
Question
We have been able to see staining in human pancreas. Do you have any suggestions? Is anti-IL7 antibody supposed to stain pancreas positively?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
From literature pancreas does express IL7. From Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-27
Question
We purchased anti-IL7 antibody for WB on sperm a few months ago. I am using human, and We intend to use the antibody for ELISA next. I would like examining sperm as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
I viewed the website and datasheets of our anti-IL7 antibody and I see that PB9521 has been tested on human in both WB and ELISA. Thus PB9521 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-10
Question
We are currently using anti-IL7 antibody PB9521 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The anti-IL7 antibody (PB9521) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
