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Product Info Summary
| SKU: | A07073-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IKB epsilon/Nfkbie Antibody Picoband®
View all IkB-epsilon Antibodies
SKU/Catalog Number
A07073-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IKB epsilon/Nfkbie Antibody Picoband® catalog # A07073-1. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IKB epsilon/Nfkbie Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07073-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IKB epsilon/Nfkbie recombinant protein (Position: Q111-D364).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07073-1 is reactive to Nfkbie in Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
52.864kDa
Background of IkB-epsilon
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon, also known as NFKBIE, is a protein which in humans is encoded by the NFKBIE gene. Predicted to be involved in D-serine transport. Predicted to act upstream of or within negative regulation of transcription, DNA-templated. Predicted to be located in several cellular components, including Golgi apparatus; nuclear lumen; and perinuclear region of cytoplasm. Is expressed in several structures, including central nervous system; dorsal root ganglion; eye; genitourinary system; and thymus. Orthologous to human NFKBIE (NFKB inhibitor epsilon).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07073-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Mouse
ELISA, 0.1-0.5 μg/ml/ml, Mouse
Positive Control
WB: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IKB epsilon/Nfkbie using anti-IKB epsilon/Nfkbie antibody (A07073-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB epsilon/Nfkbie antigen affinity purified polyclonal antibody (Catalog # A07073-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB epsilon/Nfkbie at approximately 41 kDa. The expected band size for IKB epsilon/Nfkbie is at 53 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Nfkbie (Source: Uniprot.org, NCBI)
Gene Name
Nfkbie
Full Name
NF-kappa-B inhibitor epsilon
Weight
52.864kDa
Superfamily
NF-kappa-B inhibitor family
Alternative Names
Mimecan; Osteoglycin; Ogn; Og NFKBIE IKBE NFKB inhibitor epsilon NF-kappa-B inhibitor epsilon|I-kappa-B-epsilon|NF-kappa-BIE|ikB-E|ikB-epsilon|ikappaBepsilon|nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nfkbie, check out the Nfkbie Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nfkbie: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IKB epsilon/Nfkbie Antibody Picoband® (A07073-1)
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