Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123

Product Name

Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123

View all IgD Antibodies

SKU/Catalog Number

M05183-1

Size

100ug

Form

Liquid

Description

Boster Bio Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Catalog # M05183-1). Tested in ELISA applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183-1)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM123

Isotype

Rabbit IgG

Immunogen

Human IgD

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human IgD. No cross-reactivity with human IgG, IgM, IgA, or IgE.

Reactive Species

M05183-1 is reactive to IGHD in Human

Applications

M05183-1 is guaranteed for ELISA Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42353 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 25ng/well – 200ng/well (for Capture)
0.01ug/mL – 0.1ug/mL (for Detection)

Validation Images & Assay Conditions

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Figure 1. IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgD antibody RM123

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Figure 2. ELISA result showing specificity
Sandwich ELISA using RM123 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

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Figure 3. ELISA result showing specificity
ELISA of human immunoglobulins shows RM123 reacted to the IgD from human plasma and the IgD from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Figure 4. ELISA result showing specificity
A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Gene/Protein Information For IGHD (Source: Uniprot.org, NCBI)

Alternative Names

IgD; Igh-5; Ighd; Immunoglobulin D; Immunoglobulin Delta IGHD immunoglobulin heavy constant delta constant region of heavy chain of IgD

For more info on IGHD, check out the IGHD Infographic

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In this infographic, you will see the following information for IGHD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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