Click image to see more details
Product Info Summary
| SKU: | M05183-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123
SKU/Catalog Number
M05183-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Catalog # M05183-1). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05183-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM123
Isotype
Rabbit IgG
Immunogen
Human IgD
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human IgD. No cross-reactivity with human IgG, IgM, IgA, or IgE.
Reactive Species
M05183-1 is reactive to IGHD in Human
Reconstitution
Calculated molecular weight
42353 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05183-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 25ng/well – 200ng/well (for Capture)
0.01ug/mL – 0.1ug/mL (for Detection)
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgD antibody RM123
Click image to see more details
Figure 2. ELISA result showing specificity
Sandwich ELISA using RM123 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Click image to see more details
Figure 3. ELISA result showing specificity
ELISA of human immunoglobulins shows RM123 reacted to the IgD from human plasma and the IgD from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
Figure 4. ELISA result showing specificity
A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGHD (Source: Uniprot.org, NCBI)
Gene Name
IGHD
Full Name
Immunoglobulin heavy constant delta
Weight
42353 MW
Alternative Names
Immunoglobulin heavy constant delta ;Ig delta chain C region ;Ig delta chain C region NIG-65 ;Ig delta chain C region WAH ;IGHD ; IGHD immunoglobulin heavy constant delta constant region of heavy chain of IgD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHD, check out the IGHD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 (M05183-1)
Hello CJ!
No publications found for M05183-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123
Question
We are currently using anti-Human IgD Rabbit Monoclonal antibody, Clone#RM123 M05183-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
H. Jones
Verified customer
Asked: 2016-08-05
Answer
The anti-Human IgD Rabbit Monoclonal antibody, Clone#RM123 (M05183-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-05
