Product Info Summary
SKU: | M07514-1 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA |
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Product info
Product Name
Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128
SKU/Catalog Number
M07514-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (Catalog # M07514-1). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07514-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM128
Isotype
Rabbit IgG
Immunogen
Human IgA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human IgA, including both IgA1 and IgA2. No cross-reactivity with human IgG, IgM, IgD, or IgE.
Reactive Species
M07514-1 is reactive to IGHA1 in Human
Reconstitution
Calculated molecular weight
37655 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07514-1 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection).
Validation Images & Assay Conditions
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Figure 1. IHC result
Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody RM128
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Figure 2. ELISA result showing specificity
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Figure 3. ELISA result showing specificity
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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Figure 4. ELISA result showing specificity
ELISA of human immunoglobulins shows RM128 reacted to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
Figure 5. ELISA result showing specificity
A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For IGHA1 (Source: Uniprot.org, NCBI)
Gene Name
IGHA1
Full Name
Immunoglobulin heavy constant alpha 1
Weight
37655 MW
Alternative Names
Immunoglobulin heavy constant alpha 1 ;Ig alpha-1 chain C region ;Ig alpha-1 chain C region BUR ;Ig alpha-1 chain C region TRO ;IGHA1 ; IGHA1 IgA1 immunoglobulin heavy constant alpha 1 immunoglobulin alpha 1|immunoglobulin heavy chain constant region alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGHA1, check out the IGHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 (M07514-1)
Hello CJ!
M07514-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1
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